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| Omori,Wellington P.; Pinheiro,Daniel G.; Kishi,Luciano T.; Fernandes,Camila C.; Fernandes,Gabriela C.; Gomes-Pepe,Elisângela S.; Pavani,Claudio D.; Lemos,Eliana G. de M.; Souza,Jackson A. M. de. |
| Abstract The filter cake from sugar cane processing is rich in organic matter and nutrients, which favors the proliferation of microorganisms with potential to deconstruct plant biomass. From the metagenomic data of this material, we assembled a draft genome that was phylogenetically related to Thermomonospora curvata DSM 43183, which shows the functional and ecological importance of this bacterium in the filter cake. Thermomonospora is a gram-positive bacterium that produces cellulases in compost, and it can survive temperatures of 60 ºC. We identified a complete set of biomass depolymerizing enzymes in the draft genome of Thermomonospora sp. CIT 1, such as α-amylase, catalase-peroxidases, β-mannanase, and arabinanase, demonstrating the potential of this... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Actinobacteria; Pectin; Hemicellulose; Crystalline cellulose; Orthologous genes. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100145 |
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| Melo,Ricardo Rodrigues de; Persinoti,Gabriela Felix; Paixão,Douglas Antonio Alvaredo; Squina,Fábio Márcio; Ruller,Roberto; Sato,Helia Harumi. |
| ABSTRACT Here, we show the draft genome sequence of Streptomyces sp. F1, a strain isolated from soil with great potential for secretion of hydrolytic enzymes used to deconstruct cellulosic biomass. The draft genome assembly of Streptomyces sp. strain F1 has 69 contigs with a total genome size of 8,142,296 bp and G + C 72.65%. Preliminary genome analysis identified 175 proteins as Carbohydrate-Active Enzymes, being 85 glycoside hydrolases organized in 33 distinct families. This draft genome information provides new insights on the key genes encoding hydrolytic enzymes involved in biomass deconstruction employed by soil bacteria. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Actinobacteria; Glycoside hydrolases; Streptomyces; Draft genome sequence; Soil bacteria. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400612 |
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| Rachedi,Kounouz; Zermane,Ferial; Tir,Radja; Ayache,Fatima; Duran,Robert; Lauga,Béatrice; Karama,Solange; Simon,Maryse; Boulahrouf,Abderrahmane. |
| ABSTRACT Repeated application of pesticides disturbs microbial communities and cause dysfunctions on soil biological processes. Granstar® 75 DF is one of the most used sulfonylurea herbicides on cereal crops; it contains 75% of tribenuron-methyl. Assessing the changes on soil microbiota, particularly on the most abundant bacterial groups, will be a useful approach to determine the impact of Granstar® herbicide. For this purpose, we analyzed Actinobacteria, which are known for their diversity, abundance, and aptitude to resist to xenobiotic substances. Using a selective medium for Actinobacteria, 42 strains were isolated from both untreated and Granstar® treated soils. The number of isolates recovered from the treated agricultural soil was fewer than that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial diversity; Actinobacteria; Granstar® herbicide; Resistance; 16S rRNA genes. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100079 |
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| Alves,Bruna Gomes; Martins,Cristian Marlon de Magalhães Rodrigues; Peti,Ana Paula Ferranti; Moraes,Luiz Alberto Beraldo de; Santos,Marcos Veiga dos. |
| ABSTRACT We evaluated the inclusion of two crude extracts produced by Streptomyces genus on in vitro dry matter and organic matter digestibility (IVDMD; IVOMD), cumulative gas production, volatile fatty acids (VFA), and methane (CH4) and ammoniacal nitrogen (NH3-N) concentration. The experimental design was randomized blocks with three blocks and four treatments: AMC (1.2 mg/25 mL ruminal inoculum), Caat (1.2 mg/25 mL ruminal inoculum), negative control (no inclusion of extracts), and positive control (sodium monensin, 1.7 mg/25 mL ruminal inoculum). Ruminal fluid samples were collected from three multiparous Holstein dairy cows fitted with ruminal cannula and incubated in a 24-h fermentation assay. There was no effect of crude extract inclusion in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Actinobacteria; Extracts; In vitro fermentation; Monensin. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100721 |
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| Teixeira,Manoel Araújo; Melo,Itamar Soares de; Vieira,Rosana Faria; Costa,Francisco Eduardo Carvalho; Harakava,Ricardo. |
| O objetivo deste trabalho foi realizar um levantamento da diversidade de microrganismos endofíticos, em plantas de mandioca (Manihot esculenta Crantz) coletadas de áreas comerciais, no Estado de São Paulo, e de etnovariedades dos estados do Amazonas e Bahia e, também, avaliar seu potencial para fixar N atmosférico e para produzir ácido indolacético. Nos três estados, foram identificadas 47 espécies de microrganismos pertencentes a 27 gêneros. Bacillus spp. foi o mais freqüente em todas as regiões. O maior número de gêneros foi encontrado em plantas provenientes do Estado do Amazonas, que apresenta a maior diversidade de microrganismos endofíticos. Amplificações por PCR do gene nifH foram avaliadas em espécies bacterianas pertencentes às... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Manihot esculenta; Bacillus; Actinobacteria; Fixação de nitrogênio; Ácido indolacético. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000100006 |
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| Dalitz,Camila de Araújo; Porsani,Mariana Vieira; Figel,Izabel Cristina; Pimentel,Ida C.; Dalzoto,Patrícia R.. |
| Abstract Actinobacteria occur in many environments and have the capacity to produce secondary metabolites with antibiotic potential. Identification and taxonomy of actinobacteria that produce antimicrobial substances is essential for the screening of new compounds, and sequencing of the 16S region of ribosomal DNA (rDNA), which is conserved and present in all bacteria, is an important method of identification. Melanized fungi are free-living organisms, which can also be pathogens of clinical importance. This work aimed to evaluate growth inhibition of melanized fungi by actinobacteria and to identify the latter to the species level. In this study, antimicrobial activity of 13 actinobacterial isolates from the genus Streptomyces was evaluated against seven... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Actinobacteria; Melanized fungi; Antimicrobial activity; 16S rDNA. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100032 |
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| Robles-Hernández,L; Hernández-Huerta,J; González-Franco,AC; Hernández-Rodríguez,OA; Núñez-Barrios,A; Pérez-Leal,R. |
| El chile es uno de los principales cultivos de importancia económica en México, y la marchitez causada por Fusarium es una de las enfermedades que limitan su producción. Además, el uso inadecuado de los agroquímicos en el cultivo generan problemas ambientales y de salud. Por ésto, en este estudio se evaluó la efectividad de Streptomyces sp. PRIO41 como agente de biocontrol de Fusarium spp. y como promotor del crecimiento de plantas de chile. Para ello, se determinó in vitro la patogenicidad y virulencia de Fusarium spp. en semillas de chile jalapeño, así como la interacción de los aislados fúngicos con Streptomyces sp. PRIO41 bajo 2 condiciones nutricionales. Finalmente, en invernadero se evaluó la efectividad de Streptomyces PRIO41 como (1) biocontrol de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Actinobacteria; Biofertilizante; Agente antifúngico; Biocontrol. |
| Ano: 2015 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572015000200001 |
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